FORM 3

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 INITIAL STATEMENT OF BENEFICIAL OWNERSHIP OF SECURITIES Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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(Print or Type Responses)

1. Name and Address of Reporting Person *   BOULDER VENTURES IV ANNEX LP 2. Date of Event Requiring Statement (Month/Day/Year) 01/27/2009 3. Issuer Name and Ticker or Trading Symbol ARCA biopharma, Inc. [ABIO] (Last) (First) (Middle) 1900 NINTH STREET, STE 200 4. Relationship of Reporting Person(s) to Issuer (Check all applicable) _____ Director __X__ 10% Owner _____ Officer (give title below) _____ Other (specify below) 5. If Amendment, Date Original Filed(Month/Day/Year) (Street) BOULDER, CO 80302 6. Individual or Joint/Group Filing(Check Applicable Line) ___ Form filed by One Reporting Person _X_ Form filed by More than One Reporting Person (City) (State) (Zip) Table I - Non-Derivative Securities Beneficially Owned

1.Title of Security (Instr. 4) 2. Amount of Securities Beneficially Owned (Instr. 4) 3. Ownership Form: Direct (D) or Indirect (I) (Instr. 5) 4. Nature of Indirect Beneficial Ownership (Instr. 5) Common Stock 725,111 D (1)   Common Stock 48,007 I By Boulder Ventures IV, L.P. (2)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.			SEC 1473 (7-02)
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Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)

1. Title of Derivative Security (Instr. 4)	2. Date Exercisable and Expiration Date (Month/Day/Year)		3. Title and Amount of Securities Underlying Derivative Security (Instr. 4)		4. Conversion or Exercise Price of Derivative Security	5. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 5)	6. Nature of Indirect Beneficial Ownership (Instr. 5)
	Date Exercisable	Expiration Date	Title	Amount or Number of Shares
Warrant (right to buy)	  (3)	10/10/2013	Common Stock	28,651	$ 9.7406	D (1)	Â
Warrant (right to buy)	  (3)	10/10/2013	Common Stock	1,897	$ 9.7406	I	By Boulder Ventures IV, L.P. (2)

Reporting Owners

Reporting Owner Name / Address	Relationships
	Director	10% Owner	Officer	Other
BOULDER VENTURES IV ANNEX LP 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
BOULDER VENTURES IV LP 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
BV PARTNERS IV, L.L.C. 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
Fidler Josh E 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
JONES ANDREW E 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
LEFKOFF KYLE 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
Macks Lawrence M 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â
Roshko Peter 1900 NINTH STREET, STE 200 BOULDER, CO 80302	Â	  X	Â	Â

Signatures

BOULDER VENTURES IV (ANNEX), L.P. By: BV Partners IV, L.L.C., its General Partner By: /s/ Kyle Lefkoff, Managing Member		02/05/2009
**Signature of Reporting Person		Date
BOULDER VENTURES IV, L.P. By: BV Partners IV, L.L.C., its General Partner By:/s/ Kyle Lefkoff, Managing Member		02/05/2009
**Signature of Reporting Person		Date
BV PARTNERS IV, L.L.C. By:/s/ Kyle Lefkoff, Managing Member		02/05/2009
**Signature of Reporting Person		Date
/s/ Josh E. Fidler		02/05/2009
**Signature of Reporting Person		Date
/s/ Andrew E. Jones		02/05/2009
**Signature of Reporting Person		Date
/s/ Kyle Lefkoff		02/05/2009
**Signature of Reporting Person		Date
/s/ Lawrence M. Macks		02/05/2009
**Signature of Reporting Person		Date
/s/ Peter Roshko		02/05/2009
**Signature of Reporting Person		Date

Explanation of Responses:

*	If the form is filed by more than one reporting person, see Instruction 5(b)(v).
**	Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1)	The shares are owned by Boulder Ventures IV (Annex), L.P. ("BV IV Annex") which is under common control with Boulder Ventures IV, L.P. ("BV IV LP"). BV Partners IV, L.L.C. ("BV IV LLC") serves as the sole General Partner of BV IV Annex, and has sole voting and investment control over the respective shares owned by BV IV Annex, and may be deemed to own beneficially the shares held by BV IV Annex. BV IV LLC however owns no securities of the Issuer directly. Josh E. Fidler ("Fidler"), Andrew E. Jones ("Jones"), Kyle Lefkoff ("Lefkoff"), Lawrence M. Macks ("Macks") and Peter Roshko ("Roshko") are Managing Members of BV IV LLC and share voting and dispositive power over the shares held by BV IV Annex and BV IV LP. Each of these individuals disclaims beneficial ownership of the shares reported herein, except to the extent of his respective pecuniary interest therein.
(2)	The shares are owned by BV IV LP which is under common control with Boulder IV Annex. BV IV LLC serves as the sole General Partner, and has sole voting and investment control over the respective shares owned by BV IV LP, and may be deemed to own beneficially the shares held by BV IV LP. BV IV LLC however owns no securities of the Issuer directly. Fidler, Jones, Lefkoff, Macks and Roshko are Managing Members of BV IV LLC and share voting and dispositive power over the shares held by BV IV LP. Each of these individuals disclaims beneficial ownership of the shares reported herein, except to the extent of his respective pecuniary interest therein.
(3)	Immediately exercisable.
  Remarks: This report is being filed jointly by Boulder Ventures IV (Annex), L.P., Boulder Ventures IV, L.P., BV Partners IV, L.L.C., Josh E. Fidler, Andrew E. Jones, Kyle Lefkoff, Lawrence M. Macks and Peter Roshko as of the date hereof and relates to the same transaction. Exhibit 99.1 Joint Filer Information

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